Fig. 1: Rationale and combined experimental/analysis pipeline for deep brain tumor imaging.
From: Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
a Left: Autopsy brain slice of a patient with IDH-WT glioblastoma. The corpus callosum (CC) is labeled with a dashed line. Arrowhead indicates main tumor mass; arrow indicates tumor infiltration in the CC as seen in the inset. Middle: Tumor infiltrated CC in S24 PDX mouse model. The CC is labeled with a dashed line, fluorescently labeled glioblastoma cells (GBMCs) are shown in green. Arrow indicates glioma infiltration of the CC. Right: Percentage of CC infiltration in human autopsies (n = 43 patients) and in PDX models (n = 23 PDX models). b Workflow of Deep3P. Upper left: Establishment of in vivo patient-derived glioma xenograft model. GBM cell lines are derived from patients and stably transduced with mGFP and injected into mice brain. Upper right: Combination of 2PM and 3PM to identify target regions for deep brain imaging. Bottom: Deep brain imaging in the CC and subsequent deep and machine learning based post-processing to allow simultaneous myelin, vessel and tumor analysis. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).
