Fig. 5: Glioblastoma cell polarity and vascular invasion patterns into and in the corpus callosum.
From: Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
a Top: Maximum intensity projections (MIP) of regional overviews of GBMCs in the CC and cortex. Arrowheads: exemplary cells parallel to the myelin fibers (angle <30°), asterisks: exemplary non-parallelly oriented GBMCs. Bottom left: THG signal only. Dashed: myelin fiber direction, white symbols: exemplary GBMCs aligned with white matter tracts, red symbols: exemplary non-parallelly orientated cells. Bottom right: red and white symbols represent exemplary GBMCs in the region shown above (vessel channel). b Directionality analysis of tumor cell regions in CC and cortex (n = 355 GBMCs, n = 12 experiments, n = 10 mice, 2 PDX models). Dashed: myelinated fiber direction within CC. c Rose plot of tumor cell directionality of regions in (a), in CC and cortex (n = 67 GBMCs, n = 2 experiments). d Comparison of predominant angle direction of cell polarity (n = 211 and n = 164 GBMCs in the CC and cortex, respectively, n = 13 experiments, n = 11 mice, 2 PDX models; two-sided Mann-Whitney, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). e Schematic and MIP of a GBMC (soma encircled in white) using vessels to invade into the CC. Dashed: invasion direction along blood vessel. Vessels, tumor cells and myelinated fiber shown as probability maps. f Percentage of GBMCs showing perivascular invasion into the CC as compared to within the cortex (n = 206 GBMCs, n = 14 experiments, two-sided Mann-Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). g Schematic drawing and h example of tumor cells using vessel related invasion mechanisms. Dashed: invasion direction. Vessels and tumor cells shown as probability maps. CC signal was post-processed by denoising. i Histogram of angles between perivascular cells and myelin fiber orientation. (n = 29 GBMCs, n = 8 experiments, n = 6 mice, 2 PDX models). j Histogram of blood vessel diameter of perivascular cells in CC (n = 29 GBMCs, n = 8 experiments, n = 6 mice, 2 PDX models) and cortex (n = 54 GBMCs, n = 7 experiments, n = 7 mice). PDX models: S24 and T269. Source data provided as Source Data file.
