Fig. 6: TM dynamics and neural invasion mechanisms in the corpus callosum.
From: Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
a Representative MIP time-lapse images showing TMs in the CC that use branching, protrusion, or retraction. Asterisks point at the GBMC somata, arrowheads point towards the tips of the TMs of interest. Dashed arrows: direction of the TM dynamic. b Distribution of TM dynamics in the CC and comparison with cortex using branching, protrusion or retraction (n = 163 cells from n = 14 datasets in 12 mice in 2 PDX models (S24 and T269)). c Representative MIP time-lapse images of invasion phenotypes in CC showing locomotion, translocation and branching migration. Dashed arrows: invasion direction. The straight line in the first translocation image indicates the stable location of the TM tip throughout all images. d Distribution of invasion phenotypes in the CC compared to the cortex (n = 99 invasive cells from n = 14 experiments in 13 mice, two-sided Mann-Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). e Speed comparison of invasion phenotypes in CC (n = 66 GBMCs from n = 7 experiments in 6 mice, two-sided Mann-Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). f Mean squared displacement is shown over time (n = 108 GBMCs, error bars indicate mean +/-s.e.m). Images were post-processed as probability maps and using the “smooth” function in ImageJ/Fiji in (a, c). Source data are provided as a Source Data file.
