Fig. 8: White matter reactivity in the corpus callosum upon glioblastoma invasion and colonization.
From: Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
a Representative MR images of two patients with IDH-wildtype glioma with affection of the CC (T1CE and ADC). b ROI-based quantification of apparent diffusion coefficient (ADC) on diffusion imaging (n = 8 glioma patients) in ipsi- and contralateral CC, (two-sided Mann-Whitney-Test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR and data points). c Axial MR image showing fractional anisotropy map in a glioma-infiltrated mouse brain (left) showing the CC as a region of interest (red) which is transformed out of the tract (d). Right: Zoom in CC (PDX model: S24). d Tracts of the body of CC, extracted from respective DTI image stacks and transformed into ROIs. e Mean fractional anisotropy (FA), mean diffusivity (MD) and axial diffusivity (AD), in mice MRI scans at multiple time points in CC. (n = 42 MR acquisitions (26 days (n = 5), 33 days (n = 7), 60 days (n = 12) and 73 days (n = 13) after injection)). f Regions with and without tumor infiltration analyzed in the CC. mGFP and source THG signal post-processed with denoising. g Morphological parameter (circularity) of tumor-infiltrated holes and non-infiltrated holes (n = 27 cells from n = 3 experiments in 3 mice in 2 PDX models (S24 and T269), two-sided Mann-Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). h Left: CC fibers in tumor-infiltrated regions compared to non-infiltrated regions. Images post-processed with denoising. Right: The orientation plot of fibers is shown. i Comparison of orientation of brain tumor free tissue with tumor-infiltrated regions. (n = 29 sample regions from n = 8 mice (S24 and T269), two-sided Mann-Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). j White matter displacing (left) and non-displacing (right) GBMC somata and TMs. Arrowheads show tumor-infiltrated-holes. Asterisks show a soma and a TM and their location in the corresponding THG signal showing no displacement. Images post-processed with denoising. k Comparison of a portion of displacing TMs to displacing GBMC somata (n = 33 GBMC somata and n = 22 TMs from n = 4 mice in 2 PDX models (S24 and T269), two-sided Mann–Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). Source data provided as Source Data file.
