Fig. 4: Cascading changes in a subset of epithelial cells in the tissue microenvironment (TME) of MYC-driven prostate cancer.

A UMAP of mouse prostate scRNA-seq subsetted to epithelial clusters (n = 52,116 cells, 36 samples). B UMAPs of Psca and Ly6d show cluster-enriched gene expression. C Scatter plots showing the proportion of epithelial clusters for each sample. Each dot represents a sample (n = 36) colored by lobe (anterior, dorsal, lateral, ventral). Bar represents the mean cell proportion of samples for each genotype. Statistics were generated by comparing WT (n = 20) and Hi-Myc (n = 16) samples using linear regression analysis (limma) in scRNA-seq with multiple samples (RAISIN) cell proportions test. The adjusted p-value is derived from a two-sided t-test and adjusted for multiple hypothesis testing using Benjamini-Hochberg procedure. D Heatmap showing expression of endogenous Myc, transgene MYC, and Hallmark MYC targets V1 leading edge genes from GSEA for each epithelial cluster. E Representative example of Ly6d CISH staining basal compartment in PIN gland of Hi-Myc FFPE prostate tissue. Staining was performed across 10 samples. For 10x magnification, scale bar indicates 200 μM. For 40x magnification, scale bar indicates 50 μM. F Example of KRT6A CISH staining basal compartment of intraductal carcinoma in human prostate cancer from frozen tissue sections. Staining was performed in 10 tissue punches. Scale bar indicates 500 μM.