Fig. 6: Immune cell populations enriched in the TME of MYC-driven prostate cancer.

A Immune cell clusters were subsetted from dorsal and lateral lobes of FVB/NJ mice from WT and Hi-Myc at 6 months and 10 months, and dimensionality reduction (UMAP) and clustering analysis were repeated (n = 4,474 cells, 12 samples). B Heatmap of immune cell type marker genes shows cluster-specific expression in merged scRNA-seq data. C Scatter plots showing the proportion of select immune cell populations for each sample (n = 12). Each dot represents a sample colored by genotype and age. Bars represent the mean cell proportion of samples for each group. Statistics were generated by comparing Hi-Myc samples at 6 months (n = 4) and 10 months (n = 2) using RAISIN’s cell proportions test based on linear regression modeling (limma). The adjusted p-value is derived from a two-sided t-test and adjusted for multiple hypothesis testing using Benjamini-Hochberg procedure. D UMAP of human immune cell clusters from prostatectomy scRNA-seq dataset (n = 12,108 cells, 18 samples). E Heatmap of immune cell type marker gene expression of immune cells from prostatectomy samples. Expression of F) regulatory T cell gene signature (IL2RA, FOXP3, CD4, IKZF2, CCR4, CTLA4) and G) TREM2 in human immune cell clusters.