Fig. 4: Evaluation of linearity and viral load quantification for the mNGS assay. | Nature Communications

Fig. 4: Evaluation of linearity and viral load quantification for the mNGS assay.

From: Laboratory validation of a clinical metagenomic next-generation sequencing assay for respiratory virus detection and discovery

Fig. 4

A A quantified SARS-CoV-2 PCR-positive nasopharyngeal swab from a patient with COVID-19 was serially diluted in donor nasal swab matrix and tested across 4 log10 dilutions. B A quantified HCV PCR-positive plasma sample from a patient with hepatitis C infection was serially diluted in donor plasma and tested across 4 log10 dilutions. At each dilution, the calculated mean concentration from three replicates is plotted against the expected concentration on a log scale, and the R2 correlation coefficient is determined by linear regression.

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