Fig. 4: Temporarily improved survival of bile duct ligated mice model following BVLO liver surface transplantation.

A Schematic illustration of common bile duct (CBD) ligation procedure to induce obstructive cholestasis. B Jaundice symptoms in NOG mice post-BDL, comparing non-transplanted or BVLO transplanted groups. C Survival rate of NOG mice post-BDL, comparing non-transplanted and BVLO-transplanted groups. Kaplan-Meier analysis with statistical significance assessed using the log-rank (Mantel-Cox), two-sided test, no adjustments were made for multiple comparisons. A p-value of 0.0034 was obtained. D Serum marker in BDL-ligated NOG mice: Non-Transplanted vs. BVLO transplanted. Evaluating alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct-bilirubin (D-Bil), and total-bilirubin (T-Bil), (two-tailed Mann-Whitney test. ALT, ns; p = 0.1067, AST, ns; p = 0.095, D-Bil, Statistical significance *p = 0.0311, T-Bil, Statistical significance *p = 0.0314). Error bars represent SEM. E Percentage of body weight remaining in BDL-ligated NOG mice: Non-Transplanted vs. Transplanted (Two-tailed Mann Whitney test, statistical significance *p = 0.0357). Sham day 3 (n = 5), day 5 (n = 3) and BVLO transplanted day 3 (n = 7), day 5 (n = 5); Error bars are represented as SEM. F Wholemount immunofluorescence of thick liver slices post BDL and BVLO transplantation: BVLO non-transplanted vs BVLO-Transplanted group. Scale bar, 300 µm. G Quantification of graft’ BD lumen area in BVLO-Transplanted NOG mice, BDL vs. Non-BDL (Two-tailed Mann Whitney test, Statistical significance *p-value of 0.0101). Three biological replicates were prepared and from each, three or four areas were selected in a thick section. Lumen areas were quantified using imageJ analysis software and error bars representing SEM.