Fig. 3: Loss of Mdm2 or functional impairment of Mdm2/MdmX complex decreases FA formation in a p53-independent manner.
From: Mdm2 requires Sprouty4 to regulate focal adhesion formation and metastasis independent of p53
HT1080 p53KO cells were (A) silenced for Mdm2 using siRNAs or (B) treated with 7 µM MEL23 for 24 h. A, B Immunofluorescence showing FA foci by vinculin staining (red), cell surface was outlined by phalloidin staining (green), and nuclei (blue) detected by DAPI staining of DNA. Representative images are shown above, with quantification of FA parameters below. The graphs shown represent the mean ± SD of three independent experimental replicates. For each independent experimental replicate all parameters were quantified in at least 20 events/condition for a total of at least 60 events/condition. C, D Protein levels of FA-related proteins in HT1080 p53KO cells (C) silenced for Mdm2 or (D) treated with MEL23 for 24 h. α-tubulin was used as a loading control, n = 3 samples. More details about the statistical tests used can be found in the Source Data file.
