Fig. 5: NAC90 negatively regulates plant immunity by controlling the NHP and SA biosynthetic pathways.
From: A NAC triad modulates plant immunity by negatively regulating N-hydroxy pipecolic acid biosynthesis
a–d Transcript levels of NHP (FMO1, ALD1, and SARD4) and SA (ICS1) biosynthetic genes in the leaves of 4-week-old WT, nac90-1, nac90-2, and NAC90 overexpression (NAC90-1 and NAC90-2) plants upon Psm ES4326 infection. e Chromatin immunoprecipitation-qPCR assay indicated that NAC90 directly binds to the upstream region of NHP biosynthetic genes. f Dual luciferase promoter activity assays showed that NAC90 represses the expression of NHP biosynthetic genes. g, h Abundance of NHP (g) and SA (h) in the leaves of 4-week-old WT, nac90-1, nac90-2, and NAC90 overexpression (NAC90-1 and NAC90-2) plants upon Psm ES4326 infection. i Growth of Psm ES4326 in leaves of WT, nac90-1, nac90-2, and NAC90 overexpression (NAC90-1 and NAC90-2) plants. j Symptoms of leaves treated with Psm ES4326 observed in WT, nac90-1, nac90-2, and NAC90 overexpression (NAC90-1 and NAC90-2) plants. k Transcript levels of PR1 in the leaves of 4-week-old WT, nac90-1, nac90-2, and NAC90 overexpression (NAC90-1 and NAC90-2) plants upon Psm ES4326 infection. Values showed here were the average of three biological replicates and error bars represent the SDs of three biological replicates (n = 3). Asterisks indicate significant changes compared to WT or control samples as calculated by two-tailed t test (*p value < 0.05; **p value < 0.01; ***p value < 0.001, p values are shown in the Source Data file).
