Fig. 5: Impact of OSER1 on oxidative stress in Drosophila and silkworms.
From: FOXO-regulated OSER1 reduces oxidative stress and extends lifespan in multiple species
Survival of DmOser1 mutant (a) or overexpression (OE) flies (b) fed with the standard diet with 20 mM paraquat. The number of flies in the lifespan experiment: (a) yw/DmOser1 ♀ (heterozygous control), n = 102; yw/DmOser1mt ♂, n = 98; DmOser1mt/DmOser1mt ♀ (homozygous mutant), n = 100; DmOser1mt/DmOser1mt ♂, n = 99; (b) Act-GAL4/+ ♀ (heterozygous control), n = 74; Act-GAL4/+ ♂, n = 65; Act>DmOser1 ♀ (homozygous overexpression), n = 74; Act>DmOser1 ♂, n = 62. c Control and DmOser1 overexpression fly larvae imaginal wing discs were stained with DHE (ROS indicator). d Quantification of wing disc size and DHE fluorescence intensity in the whole and different regions of wing imaginal discs. Unpaired t test, Two-tailed. Whiskers: minima and maxima, Center: median, Bounds of box: 25% and 75% percentile. e Transcripts of DmOser, Sod2, Cat, and GPx were quantified by qPCR in Drosophila larval whole wing discs. n = 6 for each group. Unpaired t test, Two-tailed. f Survival of flies overexpressing DmOser1 under conditions of starvation. Act-GAL4/+ ♀, n = 75; Act-GAL4/+ ♂, n = 68; Act>DmOser1 ♀, n = 75; Act>DmOser1 ♂, n = 72. g Survival of DmOser1 overexpressing flies under heat shock (37 °C). Act-GAL4/+ ♀, n = 75; Act-GAL4/+ ♂, n = 35; Act>DmOser1 ♀, n = 75; Act>DmOser1 ♂, n = 62. h Survival of BmOSER1 knockout silkworm larvae under heat shock (37 °C) (mixed sexes). koCtrl, n = 23; koBmOSER1, n = 18. i Expression of BmOSER1 and BmCAT mRNA in adult silkworms treated with 50 mM H2O2. Unpaired t test, Two-tailed. j Expression of BmOSER1, BmSOD1, BmCAT, and BmGpx mRNA in BmE cells after treatment with 1 mM H2O2. n = 6 for each group. Unpaired t test, Two-tailed. k Staining of silkworm embryonic BmE cells with DCF (dichlorofluorescein) with or without BmOSER1 overexpression. Unpaired t test, Two-tailed. l Expression of BmSOD1, BmCAT, and BmGpx mRNA after 1 mM H2O2 treatment in the presence or absence of BmOSER1 overexpression. Scatter plots are presented as mean with SD, and differences between control and treatment groups were analyzed by Unpaired t-test, Two-tailed. Survival curves were analyzed using Log-rank (Mantel-Cox) test. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data points derive from independent cell line lysates, Drosophila imaginal wing discs, Drosophila, and silkworms. Lifespan experiments were performed at least three times independently with similar observations. Source data are provided as a Source Data file.
