Fig. 6: OSER1 regulation of lifespan, oxidative stress, and mitochondria in nematodes.
From: FOXO-regulated OSER1 reduces oxidative stress and extends lifespan in multiple species
a Survival of control and Ceoser1 knockdown C. elegans in the presence or absence of 5 mM NAC (N-acetylcysteine). siCtrl, n = 107; siCeoser1, n = 113; siCtrl+NAC, n = 106; siCeoser1+NAC, n = 113. b Survival of control and Ceoser1 overexpressing C. elegans stimulated by 4 mM TBHP (tert-Butyl hydroperoxide). oeCtrl, n = 74; oeCeoser1, n = 77. c Quantification of 2’, 7’-dichlorofluorescin diacetate (DCF-DA) fluorescence intensity. Data are presented as mean with SD and analyzed by Unpaired t-test, Two-tailed. d Quantification of the HyPer fluorescence intensity. Data are presented as mean with SD and analyzed by Unpaired t-test, Two-tailed. Ceoser1 control or knockdown nematodes were imaged for ROS indicator gst-4p::gfp at 20 °C or 25 °C on day 1 or day 2 of adulthood (e) and quantified in (f). Data are presented as mean with SD and analyzed by two-way ANOVA. g Representative confocal image of mitochondrial morphology in control and Ceoser1 knockdown nematode strains with GFP and RFP tagged mitochondria. h Quantification of mitochondria length from (g). Data are presented as mean with SD and analyzed by Unpaired t-test, Two-tailed. i ATP levels in control and Ceoser1 knockdown nematodes. Data are presented as mean with SD and analyzed by Unpaired t-test, Two-tailed. Relative expression of Ceoser1 mRNA in various longevity mutant backgrounds, including daf-16, daf-2, eat-2, pmk-1, and an skn-1 gain‐of‐function mutant (#) cultured at 20 °C (j), and Ceoser1 mRNA expressions of glp-1 mutant cultured at 25 °C (k). Data in scatter plots are presented as mean with SD and statistically tested by One-way ANOVA (j) and Unpaired t-test, Two-tailed (k). l Survival of daf-2 mutants with normal or low levels of Ceoser1 expression. All survival curves were plotted and analyzed by Log-rank (Mantel-Cox) test using GraphPad Prism 9. daf-2 siCtrl, n = 315; daf-2 siCeoser1, n = 350. n.s., not significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All data points derive from independent nematodes. Lifespan experiments were performed at least three times independently with similar observations. Source data are provided as a Source Data file.
