Fig. 1: Recombinant SWSAP1-SWS1 form a heterodimeric protein complex.
From: The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA
A Recombinant SWSAP1-SWS1 (25, 15.5 kDa, respectively), RPA (70, 32, 14 kDa), RAD51 (37 kDa), Cy3-RAD51 (37 kDa), and Cy5-RAD51 (37 kDa) were run on SDS-PAGE gel and Coomassie stained or visualized by typhoon imaging (Cy3-RAD51 and Cy5-RAD51). B SWSAP1-SWS1 were analyzed by size exclusion and the eluted fractions were analyzed by SDS-Page gel and Coomassie staining. Experiments were performed in triplicate. C Quantitative analysis was performed to determine the size of SWSAP1-SWS1 (purple text) using known molecular standards and the partition coefficient was graphed vs the log of the molecular weight. The molecular standards used include Aprotinin (6.5 kDa), Ribonuclease A (13.7 kDa), Ovalbumin (43 kDa), Conalbumin (75 kDa), Aldolase (158 kDa), Ferritin (440 kDa), and Thyroglobulin (669 kDa) run on a Superdex200 (10/300 GL) column. Partition coefficients (Kav) of low and high molecular weight protein standards are plotted vs the Log of the MW. Experiments were performed in triplicate.
