Fig. 8: PglX is the sole specificity factor for BREX and can be rationally engineered to re-target BREX methylation and phage defence. | Nature Communications

Fig. 8: PglX is the sole specificity factor for BREX and can be rationally engineered to re-target BREX methylation and phage defence.

From: Structure and rational engineering of the PglX methyltransferase and specificity factor for BREX phage defence

Fig. 8

a EOP results of phages TB34 and Trib tested against E. coli DH5α pBrxXLSty WT and PglX mut.3 in the context of BREXSty, or against DH5α pBrxXLSty-ΔpglX with complementation plasmid pBAD30-pglX (WT) or pBAD30-pglX (mut.3). Values are mean EOPs from triplicate data, shown with standard deviation. b PacBio sequencing results showing genomic methylation in strains as described in (a).

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