Table 1 Kd (μM) values between proteins and RNAs obtained in this study

From: DMDA-PatA mediates RNA sequence-selective translation repression by anchoring eIF4A and DDX3 to GNG motifs

eIF4A1

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

0.30 ± 0.061

26 ± 17

2.0 ± 0.50

0.41 ± 0.053

12 ± 1.2

1.5 ± 0.34

(UC)10

ND

ND

 

28 ± 5.1

2.8 ± 0.24

  

eIF4A1 Phe163Leu-Ile199Met

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

ND

     

eIF4A2

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

1.6 ± 0.17

     

DDX3X (helicase core)

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

0.80 ± 0.12

 

29 ± 61

0.71 ± 0.087

  

(UC)10

ND

ND

 

ND

ND

  

DDX3X Gln360Leu (helicase core)

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

5.1 ± 2.3

     

DDX3X Gln360Pro (helicase core)

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

ND

     

DDX6 (helicase core)

 

ADP + Pi

AMP-PNP

ATP

RNA

DMSO

DMDA-PatA

iPr-DMDA-PatA

DMSO

DMDA-PatA

DMSO

DMDA-PatA

(AG)10

ND

ND

 

1.8 ± 0.72

2.6 ± 0.71

  
  1. The fluorescence polarization of FAM-labeled RNAs was determined and fitted to the Hill equation to determine the Kd. ND, not determined.
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