Fig. 2: Structural comparison of conserved motifs in the inactive and active FZD₇ conformations.

The intracellular rearrangements of conserved residue a P481^6.43, b extended molecular switch residues, F474^6.36 - W547^6.36, e Y478^6.40 and f molecular switch residues W547^7.55 - R470^6.32 are observed upon agonist binding. c A network of extended polar contacts in ICL3 is made between the two conformations of FZD₇. d Comparison of the nucleotide-free Gα_s-bound FZD₇ (pink, PDB: 7EVW) and inactive FZD₇ (purple) highlighting structural changes of conserved residues and regions of interest. g G protein binding rearranges residues in ICL1 involving D278^1.57, F282, R281, R280, and M279. Scatterplot of occurring χ1 χ2 dihedral angles of residue h W354^3.43 and i W547^7.55 calculated for each frame (50 ps time steps) over the trajectory from replica 1 of FZD₇ simulations of active and inactive state.