Fig. 4: Water-mediated interactions and conserved residues of FZD₇.

a Overall organization of the internal water pocket of inactive FZD₇. b The region of the cavity referred to as the bottleneck is comprised of the following residues: L415^ECL1, D405^ECL1, Y489^6.51, Y534^7.42, and K533^7.41. c Phylogenetic analysis of FZD₇ depicting highly conserved and interacting residue pairs involved in the internal water pocket. d, e, f The tight base of the internal water cavity facilitates a reorientation of residues W354^3.43, Y478^6.40, Y296^2.51, and V540^7.48 in the d inactive conformation and e active conformation. f A top view of the transmembrane domains between the two structures highlighting residues of interest.