Fig. 5: MK-derived platelets contain particles inherited from their parental MKs. | Nature Communications

Fig. 5: MK-derived platelets contain particles inherited from their parental MKs.

From: Lung megakaryocytes engulf inhaled airborne particles to promote intrapulmonary inflammation and extrapulmonary distribution

Fig. 5

a Immunofluorescent images of CD41+ platelets from peripheral blood of mice exposed to 2 mg/kg body weight Cy5-PSs for 72 h show platelet colocalized with fluorescent particles (red). b Schematic shows o.p.a. transplantation of 1.0 ×107 platelets and 50 ng Cy5-PS mixture into c-mpl-/- mice. c Fluorescent images of liver sections from recipient c-mpl-/- mice in b 72 h after transplantation showing Cy5-PSs adhered onto platelets (Green). d Fluorescent images of lung sections in recipient mice showing platelets (green) and Cy5-PSs (red) inside CD45+ immune cells (yellow) at 72 h post platelets-Cy5-PS mixture transplantation. e Intravital two-photon imaging shows lung MK (green) releasing nascent platelets containing Cy5-PSs (pink). Red dotted square is magnified in bottom right. f Fluorescent images of Dami cells treated with 200 ng/mL Cy5-PSs for 12 h showing endoplasmic distribution of engulfed particles. Phalloidin-stained cytoskeleton (green, left panel), carbocyanine-stained membrane (red, right panel) and engulfed fluorescent particles. g Continuous imaging analysis of MKs every 3 min. Nascent platelet clusters released from Cy3-labeled MKs (yellow) are shown. h Flow cytometry images of Cy5-PSs (red)-laden platelets (CD41+, green) collected from MK induction medium. Figure 5b, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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