Fig. 6: Extrapulmonary transportation of inhaled particles through MK-derived platelets. | Nature Communications

Fig. 6: Extrapulmonary transportation of inhaled particles through MK-derived platelets.

From: Lung megakaryocytes engulf inhaled airborne particles to promote intrapulmonary inflammation and extrapulmonary distribution

Fig. 6

a Schematic shows o.p.a. transplantation of particle-laden CD41+GFP+ MKs into c-mpl-/- mice for tracking platelet distribution. b Fluorescent images of lung sections from recipient c-mpl-/- mice show MKs (CD41+GFP+DAPI+) remain loaded with Cy5-PSs (red) 72 h after transplantation. c Immunofluorescent images of liver sections from recipient c-mpl-/- mice 72 h after MK transplantation. Donor MK-derived platelets (green) overlap with Cy5-PSs (red). d Ratio of platelet-bound and platelet-free particles in liver, spleen and kidney determined by RFI analysis of fluorescent images (n = 6 randomly captured images). e Fluorescent images show donor-derived platelet (green) in the liver is transported through blood vessel (Alexa Fluro 647-dextran+, circled out with white dotted line). f Immunofluorescent images of EVs (CD63+, yellow) and Cy5-PS (red) in liver sections from recipient c-mpl-/-mice 72 h after MK transplantation. CD41+GFP+ donor MK-derived platelets (green) with engulfed Cy5-PSs (red) are shown in the bottom as reference. g Fluorescent images of liver sections from WT and c-mpl-/- mice after exposure to Cy5-PSs for 72 h. Fewer particles are seen in c-mpl-/- mice. h ICP-OES analysis of SiO2 content in the lung, liver, kidney, spleen and peripheral blood of WT and c-mpl-/- mice treated with 2 mg/kg body weight SiO2 particles for 72 h (n = 8 mice per group). i Regression analysis of the particle content in the liver based on RFI quantification and ICP-OES method. Dotted lines represent 95% confidence intervals for the linear regression line. Each dot corresponds to one individual mouse sample. Statistical analysis was performed by linear regression analysis, two-side Pearson correlation test. j Counts per field of TUNEL positive cells in liver sections from WT and c-mpl-/- mice at 24 and 72 h after CB exposure (n = 3 mice per group). Box and whisker plots indicate median, interquartile range and 10th to 90th percentiles of the distribution (d, h). Data are presented as the mean values of 3 independent experiments ± SEM (d, h and j), P-value by one-way ANOVA (d, h and j). N.S, not significant. Figure 6a, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.

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