Fig. 1: The minimal type I-F2 CRISPR-Cas system. | Nature Communications

Fig. 1: The minimal type I-F2 CRISPR-Cas system.

From: Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells

Fig. 1

a Schematic representation of the Moraxella osloensis strain CCUG 350 type I-F2 CRISPR-Cas system showing Mos350 Cascade stoichiometry and crRNA processing. The genes comprising the Mos350 Cascade complex are represented in different colors. Scale bars show the gene sizes of Cas3 and Cascade (including intergenic regions). Repeats and spacers in the CRISPR array are indicated using black diamonds and red rectangles, respectively. Cas6 cleaves pre-crRNA transcripts at the positions indicated by the blue arrow in the repeat sequences to produce mature crRNAs. b The identified I-F2 Cas7 proteins were subjected to phylogenetic analysis using the maximum likelihood method, along with Cas7 proteins from other type I systems. The scale bar represents the number of substitutions per site. Source data are provided as a Source data file.

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