Fig. 6: Cascade-ABE8e has a wide editing window with a bimodal distribution.

a Schematic illustration of gene structures of 5NABE, 5CABE, 7NABE, and 7CABE. TadA-8e was fused to the N-terminus or C-terminus of Cas5 or Cas7. Orange: Cas7; Dark Yellow: Cas6; Cyan: Cas5; Pale blue: TadA-8e; Pink: T2A; Green: EGFP. b The editing window of the 5NABE base editor. The pink area marks the PAM of site 1 and the pink line labels the protospacer. The blue area marks the PAM of site 2 and the blue line labels the protospacer. c A∙T-to-G∙C editing efficiency of 5NABE at three endogenous genomic loci with multiple adenines (sites 3–5). d A∙T-to-G∙C editing efficiency of 5NABE at six different genomic loci (sites 6–11) in HEK293T cells using different fluorescence thresholds (FITC). e A∙T-to-G∙C editing efficiency of 5NABE at the BCL11A enhancer (GATA1 binding site) in HEK293T cells. The pink area marks the PAM of site 12, and the blue area labels the GATA1 binding site. Non-Target: Only the plasmid expressing the Cascade-TadA8e was transfected. Target: The plasmid expressing the Cascade-ABE and the plasmid expressing the mini-CRISPR were co-transfected. All values in (b–e) are represented as mean ± s.d. (n = 3 biological replicates), except for non-target results at site 5 of two biological replicates (c). Source data are provided as a Source data file.