Fig. 7: Off-target effects of 5NABE. | Nature Communications

Fig. 7: Off-target effects of 5NABE.

From: Engineered minimal type I CRISPR-Cas system for transcriptional activation and base editing in human cells

Fig. 7

a Left: Potential Cascade-dependent off-target sites of 5NABE when targeting site 10 in HEK293T cells. Left: The pink area marks the PAM motif and the blue area marks the protospacer of site 10. The gray area indicates that complementary pairings are not required. Lowercase letters represent mismatched bases compared to the target sequence. Right: Base editing efficiency of 5NABE at target (data from Fig. 6d) and off-target sites. b Left: Potential Cascade-dependent off-target sites of 5NABE when targeting site 5 in HEK293T cells. Left: The pink area marks the PAM motif and the blue area marks the protospacer of site 5. The gray area indicates that complementary pairings are not required. Lowercase letters represent mismatched bases compared to the target sequence. Right: Base editing efficiency of 5NABE at target (data from Fig. 6c) and off-target sites. OT ID: Off-target site ID. Non-Target: Only the plasmid expressing the Cascade-TadA8e was transfected. Target: The plasmid expressing the Cascade-ABE and the plasmid expressing the mini-CRISPR were co-transfected. All values were mean ± s.d. (n = 3 biological replicates), except for non-target results at target site 5 (b) of two biological replicates. Source data are provided as a Source data file.

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