Fig. 2: Binding of 7g7 on PvCelTOS.
a Crystal structure of PvCelTOS dimer in complex with 7g7 Fab. The dimer of PvCelTOS is in black/gray ribbons; the heavy and light chains of 7g7 Fab are in blue and light blue ribbons. Superposition of PvCelTOS b monomer and c dimer from the current study (black/gray) with the previously reported PDB:5TSZ21 (wheat). d Full-length sequence of PvCelTOS. Helix and loop regions are labeled. Binding interface residues are in blue. e Binding epitope of 7g7 on PvCelTOS. The PvCelTOS monomer is represented in gray ribbon; the binding interface is highlighted in blue. f Mapping of epitope within the loop regions using ELISA. Each PvCelTOS mutant carries consecutive 3-4 residues mutated to alanine and the proteins were purified for ELISA to examine their interaction with 7g7. The graph is shown as mean ± s.d. of three biological replicates. g Binding affinity of 7g7 to PvCelTOS as determined by BLI. Representative sensorgram showing association and dissociation when 7g7 is immobilized on biosensors. Black lines are sensograms for two-fold dilution series of PvCelTOS. Blue lines are fitted curves correspond to a 1:1 model. The KD value is the mean ± s.e.m. of three biological replicates.
