Fig. 7: Protective immunity of 7g7 and 4h12 in BALB/c mice.
Liver stage infectivity in mice challenged with chimeric P. berghei sporozoites expressing P. falciparum CelTOS or P. vivax CelTOS. a Pb-PfCelTOS liver burden among mice immunized with 7g7 and 4h12 mAb showed lower parasite load (RLU values) compared to naïve mice (no antibody control). The Anderson‒Darling test for normality confirmed a normal distribution for Pb-PfCelTOS responses. Therefore, significance differences were determined by One-way ANOVA with a Tukey multiple comparison. Error bars represent mean ± s.d. from ten mice. c Similar results with lower parasite liver loads were also observed for Pb-PvCelTOS liver burden among mice immunized with 7g7 and 4h12. The Anderson‒Darling test for normality did not confirm a normal distribution for Pb-PvCelTOS responses. Therefore, significant differences were determined by Kruskal‒Wallis with a Dunn’s multiple comparison analysis. Antibody isotype matched control group was done using anti-PvCSP VK247 antibody 2E10.E938. Error bars represent mean ± s.d. from ten mice. b, d The Kaplan‒Meier curve showing the prepatent period of Pb-PfCelTOS and Pb-PvCelTOS sporozoites in BALB/c mice that were passively immunized with 7g7 and 4h12 mAbs. Prepatency is defined as the time to 0.1% parasitemia and statistical significance between the survival curves was assessed using the Log-Rank (Mantel‒Cox) Test (n = 10).
