Fig. 1: Repetitive swim stress alters circadian clock gene expression in the mouse mPFC. | Nature Communications

Fig. 1: Repetitive swim stress alters circadian clock gene expression in the mouse mPFC.

From: Prefrontal cortex molecular clock modulates development of depression-like phenotype and rapid antidepressant response in mice

Fig. 1

a Schematic overview of the experimental design: CDM paradigm (5 days of 10 min swimming), tissue harvesting time points and gene expression analyses. b Relative mRNA expression of clock genes Per1, Per2, Cry1, Cry2, Bmal1, Npas2, Rorα, Rev-erbα, Rorβ, Rorγ normalized to s12, ApoE and GAPDH in mPFC samples harvested from naive (control) and CDM mice every 4 h at 12:12 h LD condition (n = 5 mice per group, two-way ANOVA: P values of the CDM effect is displayed inside the graph; Bonferroni post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001 control vs. CDM). c Amplitude of rhythmic expression and acrophase (n = 30 mice: 5 ×6 ZT points; Extra sum of squares F test: **P < 0.01, ***P < 0.001). d Schema summarizing the effects of stress on clock gene expression in mPFC–CDM potentiates Per2 and Cry2 expression, while decreases Rors level. Data are presented as mean ± SEM. See also Supplementary Fig. 1 and Supplementary Data 1. Source data are provided as a Source Data file.

Back to article page