Fig. 4: Specific knockdown of Per2 in mPFC elicits antidepressant effects. | Nature Communications

Fig. 4: Specific knockdown of Per2 in mPFC elicits antidepressant effects.

From: Prefrontal cortex molecular clock modulates development of depression-like phenotype and rapid antidepressant response in mice

Fig. 4

a Schematic overview of the experimental design: CDM paradigm, followed by stereotactic microinjections of Accell siRNA and test phase. b Relative mRNA expression of Per2 in mPFC at ZT06 (n = 6 siCntr, n = 7 siPer2-injected mice, two-tailed Student’s t test: **P < 0.01). c Representative western blot (right) and quantitative data (left) of Per2 protein expression normalized to Histone 2B (H2B) in mPFC at ZT06 (n = 4 mice per group, two-tailed Student’s t test: *P < 0.05). d Immobility time during test phase TST of siCntr (n = 7) and siPer2 (n = 8) injected mice, two-tailed Student’s t test: *P = 0.0431). e Immobility time during test phase FST of siCntr (n = 7) and siPer2 (n = 8) injected mice, two-tailed Student’s t test: *P = 0.0231). f Relative mRNA expression of Homer1a at ZT06 in mPFC of siCntr (n = 6) and siPer2 (n = 7) injected mice (two-tailed Student’s t test: *P < 0.05). g Schema summarizing the effects of Per2 knockdown in mPFC on Homer1a expression and depression-like behavior. Data are presented as mean ± SEM and the individual data points are depicted. See also Supplementary Fig. 4 and Supplementary Data 1. Source data are provided as a Source Data file.

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