Fig. 5: REV-ERBα modulates depression-like phenotype and Homer1a expression via Bmal1. | Nature Communications

Fig. 5: REV-ERBα modulates depression-like phenotype and Homer1a expression via Bmal1.

From: Prefrontal cortex molecular clock modulates development of depression-like phenotype and rapid antidepressant response in mice

Fig. 5

a Experimental design schematic. b Immobility time during CDM induction and test phase FST of WT mice acutely i.p. injected with vehicle/Rev-ERB agonist SR10067 (30 mg/kg) after the swim on day 1 at ZT06 (n = 10, repeated measures two-way ANOVA with Bonferroni post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001). c Immobility time during test phase TST (n = 10 mice, two-tailed Student’s t test: *P = 0.0267). d Sucrose preference assessed in IntelliCage (n = 10 mice, repeated measures ANOVA with Bonferroni post hoc test: **P < 0.01 and #P < 0.05, ###P < 0.001 vs baseline). e Relative Bmal1 and Homer1a mRNA expression at ZT06 in mPFC of vehicle/SR10067 injected WT mice (n = 5, two-tailed Student’s t test: *P < 0.05, **P < 0.01). f Experimental strategy: 1 week after CDM, WT mice are vehicle/SR10067 and saline/ketamine injected 6 h prior to test. g Immobility time during test phase FST (n = 6 mice, two-way ANOVA with Bonferroni post hoc test: ###P < 0.001 vs saline). h Immobility time during test phase TST (n = 6 mice, two-way ANOVA with Bonferroni post hoc test: ###P < 0.001 vs saline). i Schema summarizing the effects of Rev-ERB activation on Bmal1/Homer1a expression. j Experimental design schematic. k Immobility time during induction and test phase of mPFC CaMK2a-Bmal1KO mice acutely injected with vehicle/SR10067 on day 1 at ZT06 (n = 7 mice, repeated measures ANOVA with Bonferroni post hoc test: #P < 0.05, ##P < 0.01, ###P < 0.001 vs day 1; *P < 0.05 day 5 vs test phase). l Immobility time during test phase TST (n = 7 mice, two-tailed Student’s t test). m Relative mRNA expression of Homer1a at ZT06 in mPFC of vehicle/SR10067 injected mPFC CaMK2a-Bmal1KO mice (n = 4 mice, two-tailed Student’s t test). n Immobility time during induction phase of WT (n = 8) and Rev-erbαKO mice (n = 6) (repeated measures two-way ANOVA with Bonferroni post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001). o Immobility time during test phase FST and TST (n = 8 & 6 mice, two-tailed Student’s t test: ***P = 0.0005 and *P = 0.0119). p Relative mRNA expression of Bmal1 and Homer1a at ZT06 in mPFC of WT (n = 8) and Rev-erbαKO mice (n = 6) (two-tailed Student’s t test: *P < 0.05, ***P < 0.001). Data are presented as mean ± SEM and the individual data points are depicted. See also Supplementary Fig. 5 and Supplementary Data 1. Source data are provided as a Source Data file.

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