Fig. 5: P2KOad mice show higher sensitivity to CL316,234-induced EE at thermoneutrality. | Nature Communications

Fig. 5: P2KOad mice show higher sensitivity to CL316,234-induced EE at thermoneutrality.

From: Adipocyte deletion of the oxygen-sensor PHD2 sustains elevated energy expenditure at thermoneutrality

Fig. 5

A P2KOad (KO, red, n = 3) and control (C, blue, n = 3) mice were challenged with CL316,243 i.p. (CL; 1 mg/g BW) at RT (21 °C). Then switched to TN housing (28 °C) and administered second CL i.p. CL given under TN conditions led to higher energy expenditure in P2KOad mice, B similar activity levels in both genotypes, C lower respiratory exchange ratio in P2KOad mice, D higher sensitivity to CL-induced NEFA release in P2KOad mice, E higher CL-induced Ucp1 mRNA expression in BAT and iWAT in P2KOad mice and F higher CL-induced UCP1 protein levels in P2KOad BAT. Data are presented as mean + /- SEM. *P < 0.05, **P < 0.01 by Student t test (two-tailed) or one-way ANOVA (E). For EE, ANCOVA was performed with body weight as co-variant. A Experimental design image was created with BioRender.com released under a Creative Commons Attribution-Non Commercial-NoDerivs 4.0 International license. Source data and uncropped blots are provided as a Source Data file.

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