Fig. 1: Concept for following the transformation reaction of reconfigurable DNA origami array structures on the single-molecule level in real time.
From: Controlled mechanochemical coupling of anti-junctions in DNA origami arrays
a Scheme of the DNA origami array model structure transforming upon addition of DNA trigger strands. Red and green FRET probes (ATTO647N-IowaBlack RQ and ATTO542-BHQ2, red/ black and blue/ purple circles) are placed at the positions at which the transformation reaction is studied. The transformation process occurs diagonally, starting either from the top right corner, as shown in the sketch, or from the bottom right corner. b Sketch of conformational flipping of a single anti-junction. Blue DNA strands represent sections of the scaffold strand, whereas pink and orange strands represent different staple strands. c, d AFM images of the DNA origami array (c) before and (d) after overnight incubation with trigger strands indicate a successful transformation of the structure. e, f Exemplary TIRF images of the DNA origami array structure before and after incubation with and without trigger strands. Fluorescence of ATTO647N is shown in red, fluorescence of ATTO542 in blue and co-localized fluorescence of both in white.
