Fig. 2: Real-time imaging of the transformation reaction of DNA origami arrays.

a Representative single-molecule fluorescence intensity transients of DNA origami array with a green and a red FRET probe incorporated after addition of five trigger DNA strands at 0 s. The time the transformation occurs at the red and the green FRET probe positions is marked with an arrow. Fluorescence of ATTO647N and ATTO542 is shown in red and blue, respectively. b Transformation time after the addition of five trigger DNA strands at the position of the red FRET probe (red) and the green FRET probe (blue). c Scheme of the different positions used for the placement of the FRET probes on DNA origami arrays for tracking the transformation reaction. d–g Time difference between the transformation occurring at the positions of the green and red FRET probes for different FRET probe positions. h Proposed, simplified energy landscape of the transformation reaction. The intermediates at which the studied positions switch their conformation are marked with numbers.