Fig. 1: Fatty acid synthesis is a feature of reparative macrophages.

a Tissue injury models included in the analysis. b The scRNA-seq analysis of skin wound macrophages of phase I (1 dpi) and phase II (6 dpi). UMAP highlights wound healing phases. c GO enrichment analysis showing the highly enriched metabolic pathway associated with phase I and phase II. Pie charts represent enriched biological processes and the ratio of gene number that enriched in each group, small dots represent genes associated with the processes. The size of each pie indicates the number of genes enriched in a specific term. d The mRNA expression of lipid metabolism-associated genes in sorted wound macrophages isolated at 1 and 6 dpi (n = 3 mice per group). e Heatmaps showing ssGSEA scores of phase I and phase II macrophages using signature genes associated with FAO (mmu00071) and FAS (mmu00061) pathways. f Developmental trajectory of skin wound macrophages. Sample are colored by wound healing phases or macrophage subtypes. g Heatmap showing expression patterns of glycolysis-, tricarboxylic acid cycle (TCA)-, OXPHOS-, pentose phosphate pathway (PPP)-, FAO-, and FAS-associated signature genes among macrophage subtypes from GSE131364 dataset. Unless specified otherwise, the data are presented as means ± s.e.m. (error bar) and compared using the two-tailed Student’s t test. Source data are provided as a Source Data file.