Fig. 2: Identification of 12 antiphage systems with TIR domains in E. coli.
From: The role of TIR domain-containing proteins in bacterial defense against phages
a The domain organization of each system was shown, and their defense activities were determined in triplicate using E. coli MG1655 against 111 different phages as described in Methods. The color indicates the fold reduction in EOP of the representative results of each TIR system against each phage in EOP experiments. The bar graph shows the number of phages restricted in each system. b Essential domain architectures and mutational analysis of the defense systems. Conserved amino acids or the putative active sites were indicated with red stars. Assays were done in triplicate and data were presented as the mean ± S.D. E. coli MG1655 cells containing the empty vector were used as controls. Asterisks indicate statistical significance relative to the control. *, **, ***, and **** indicate p < 0.05, p < 0.01, p < 0.001, and p < 0.0001, respectively (ANOVA). The ns indicates not significant. Source data are provided as a Source data file.
