Fig. 6: Treatment with Nb-Fc selectively inhibits neutrophil migration.

a–c Nb-Fc supressed neutrophil migration in vivo. Mice were intraperitoneally injected with Nb-Fc or Iso-Fc, followed by injecting thioglycolate. The peritoneal lavage fluid was collected 4 h after injection. Flow cytometry was used to analyze cell types in the peritoneal lavage fluid. Ly6G⁺CD11b⁺ cells were identified as neutrophils. a Representative flow cytometric plots of peritoneal exudate cells (PECs) stained with antibodies against Ly6G and CD11b. Quantification of PECs (b, n = 8) and neutrophils (c, n = 8) in the peritoneal lavage fluid. d–i Nb-Fc inhibited neutrophil migration in vitro. White blood cells (WBCs) from were isolated from human and murine whole blood and used for migration assessment by transwell assay analysis. WBCs were placed in the upper chamber, followed by adding Nb-Fc or Iso-Fc (50 μg/mL). fMLP (2 μM) was added in the lower chamber to induce neutrophil migration. The migrated neutrophils in lower chamber were measured by flow cytometry. Representative flow cytometric plots of (d) murine and (g) human WBCs in the lower chamber of transwell. Counts of mouse neutrophils (Ly6G⁺CD11b⁺, e) and human neutrophil (CD45⁺CD16⁺CD11b⁺, h) in lower chamber (n = 6). Counts of (f) mouse or (i) human cell types other than neutrophils (non-neutrophil leukocytes) in lower chamber (n = 6). Data are presented as mean ± SEM. Unpaired two-sided Student’s t test was used (b, c, e, f, h, i). Source data are provided as a Source Data file.