Fig. 1: Ribosome biogenesis inhibition kinetics of usnic acid.
From: The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation
a Chemical structure of (+)-usnic acid (triketone moiety highlighted in blue). b Minimum inhibitory concentration (MIC) of usnic acid for the yeast wildtype strain C303a grown in minimal media. Early logarithmic cells were diluted to OD600 0.01 and grown in microtiter plates in a Bioscreen device under continuous shaking and automated OD600 measurements every 30 min. The plotted values reflect the OD600 after 24 h growth in the presence of different concentrations of usnic acid. Mean and standard deviation of two biological replicates each measured in technical triplicates. c Kinetics of nuclear Rpl7a-GFP accumulation upon usnic acid treatment. The Rpl7a-GFP reporter strain41 was treated with 40 µM usnic acid and monitored by fluorescence microscopy in a time series (0–5 min). Source data are provided as a Source Data file for b and c.
