Fig. 2: Usnic acid shows different Rpl7a-GFP accumulation kinetics in the nucleus than diazaborine and leads to fragmented nucleolar Nog1-GFP signals.
From: The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation
a The Rpl7a-GFP reporter strain41 was treated with diazaborine (10 µg/ml) or usnic acid (40 µM) and the GFP signal was monitored via fluorescence microscopy. b Wildtype yeast cells expressing the chromosomally GFP-tagged shuttling factor Nog1 and the mCherry-tagged nucleolar factor Nop58 were treated with usnic acid (40 µM), diazaborine (10 µg/ml) or both inhibitors. Untreated cells served as control. Source data are provided as a Source Data file.
