Fig. 2: Integrative transcriptome profiling of gill, intestinal, and blood atlases.

a UMAP visualization of cell type distribution after integration of the gill, blood, and intestinal atlases. The color annotation indicates each cell type annotated. b Heatmap of selected marker genes across various cell types. c Feature plot of selected marker genes across various cell types. d The top bar plot shows the distribution of all cell types in each sample. The color annotation indicates various samples. The bar plot in the middle shows the distribution of all cell types in three tissues. The bottom bar plot shows the cell numbers of each cell type. e Immunohistochemistry examinations of the gill filaments and typhlosoles. Numerous VLRA⁺ cells from the gill filaments and typhlosoles were well recognized by anti-VLRA pAb with experiments performed at least three times. f Heatmap showing the similarity of multiple cell types in three organs/tissues. The color of the outermost rectangles indicates various cell types. The color of rectangles on the second floor indicates gill, blood, or intestine. The brighter color in the picture, the higher AUROC value is. The AUROC value is proportional to the cell correlation. T-like cells and B-like cells are indicated in the red box. g Heatmap of the marker genes and top 20 DEGs in T-like cells and B-like cells across three tissues.