Fig. 7: Analysis of MPL-L+ T-like cell transition states.

a Developmental pseudotime trajectory of five T-like cell subpopulations. 2D pseudotime plot showing the dynamics of five T-like cell subpopulations (upper panel). The color indicates the differentiation degree of the five cell types (lower left panel). The deeper color indicates the early state. The color of the bottom right figure shows three branches of those states (lower right panel). b The ridge plot shows the distribution of cell density by five T-like cell subpopulations during the transition (divided into 3 states), along with the pseudotime. c The figure shows the change in VLRA gene expression in five T-like cell subpopulations over pseudotime with cell proportion expressing VLRA on the x-axis and its average expression on the y-axis. d The diagram shows the cell transitions of T-like cell subpopulations along with the pseudotime. e Developmental pseudotime trajectory of MPL-L+ T-like cells across gills, blood, and intestines with the top figure color-coded by tissue and the bottom by differentiation level. f Bar plot displays the proportion of cells that expressed the VLRA gene (nUMI > 0) in MPL-L+ T-like cells of gills, blood, and intestines. g Heatmap of gene expression arranged along the pseudotime trajectory. h Pseudotime plots of selected genes. i The figure shows the change in MPL-L gene expression in MPL-L+ T-like cells in three tissue types over pseudotime with cell proportion expressing MPL-L on the x-axis and its average expression on the y-axis. j Scatterplot on the left displays correlations of MPL-L expression with JAK2 expression in MPL-L+ T-like cells. The scatterplot on the right displays correlations of MPL-L expression with JAK2 expression in MPL-L+ T-like cells across gills, blood, and intestines. For correlation analysis, gene expression data were imputed by MAGIC. Utilizing Spearman’s rank correlation coefficient to measure the strength and direction of the association between the two variables. The statistical test used was two-sided. k Scatterplot on the left displays correlations of MPL-L expression with STAT5B expression in MPL-L+ T-like cells. The scatterplot on the left displays correlations of MPL-L expression with STAT5B expression in MPL-L+ T-like cells across gills, blood, and intestines.