Fig. 2: FoxO TFs translocate into the nucleus upon exit from naïve pluripotency.
From: FoxO transcription factors actuate the formative pluripotency specific gene expression programme
a Confocal microscopy images after IF, showing FOXO1 (purple) and ESRRB (green) in WT and Pten KOs in 2i and at N24. DAPI staining is shown in blue. One representative image from n = 3 independent experiments is shown. Scale bar = 20 μM. b Quantification of FOXO1 and ESRRB nuclear intensity (in arbitrary unit, arb. units) measured from confocal images of WT cells in 2i (n = 135) and 8 h (N8, n = 136), 16 h (N16, n = 88) and 24 h (N24, n = 127) after 2i withdrawal. Data were obtained from n = 2 independent experiments. p values are derived from a two-tailed Wilcoxon rank sum test. c Quantification of FOXO1 nuclear intensity (arb. units) based on images of WT (n = 189 [2i] and n = 164 [N24]) and Pten KO (n = 100 [2i] and n = 153 [N24]), as in (a). Data were obtained from n = 2 independent experiments. p values were calculated as in (b). d Confocal microscopy images after IF showing FOXO1 (white), SOX2 (green), GATA4 (red), or OTX2 (red) in WT E4.5, E4.75 and E5.5 embryos. Hoechst staining is shown in cyan. One representative image of n = 3 independent experiments is shown. Scale bar = 100 μM. e Quantification of FOXO1 nuclear intensity (arb. units) measured from confocal images of WT epiblast from E4.5 (n = 12), E4.75 (n = 12) and E5.5 (n = 8) embryos, as in (d). Data were obtained from n = 3 independent experiments. Indicated p values are derived from a two-tailed Wilcoxon rank sum test.
