Fig. 5: Interference with FoxO1 nuclear shuttling impairs the transition from the naïve to the formative GRN.
From: FoxO transcription factors actuate the formative pluripotency specific gene expression programme
a Flow cytometry analysis of Rex1-GFP levels in WT cells transfected with control (siGFP and siScr, grey) or siRNAs targeting FoxO1 (siFoxO1, red). One representative of n = 3 independent experiments is shown. b Volcano plot showing RNA-seq data of WT cells at N24, treated with siRNA against FoxO1. DEGs (p adj. ≤ 0.2) that are bound by FoxO1 are colour coded depending on whether they are bound only in 2i (green), only at N24 (blue) or in both conditions (purple). Selected naïve and formative genes are indicated in the plot. For each quadrant, percentages (%) of FoxO1 2i-only, N24-only and 2i&N24 targets are indicated. c CUT&RUN analysis of H3K27ac levels on indicated enhancers after FoxO1 siRNA treatment. Signal was normalised to a genomic background region that did not exhibit any H3K27ac signal in WT cells. Data were further normalised to a H3K27ac peak found in Drosophila spike-in cells. Data are shown as relative to siScr control. n = 2 biological replicates. An independent experiment with further n = 2 biological replicates shown in Supplementary Fig. 5g.
