Fig. 3: GmSW17, GmSGF11 and GmENY2 could form DUBm, which affects the seed size.
From: Natural variation in GmSW17 controls seed size in soybean
a Interaction between different haplotypes of GmSW17 and GmSGF11 in a yeast two-hybrid assay. Transformed yeast cells are cultured on DDO (double dropout -Trp/-Leu) or QDO (quadruple dropout -Trp/-Leu/-His/-Ade) synthetic dropout medium. The number at the top indicates the different serial dilutions. AD, GAL4 activation domain. BD, GAL4 DNA-binding domain. b Co-IP analysis of the protein interactions between different haplotypes of GmSW17 and GmSGF11, using anti-Flag beads. c Interaction between GmENY2 and GmSGF11 in a yeast two hybrid assay. d Co-IP analysis of the interactions between GmENY2 and GmSGF11, using anti-Flag beads. e Comparison of seed width between DN50 and GmSGF11-OE lines. Scale bars, 1 cm. f Seed width of DN50 and GmSGF11-OE lines (n = 120, 30 biological independent plants for DN50 and GmSGF11-OE lines, respectively). g 100-seed weight of DN50 and GmSGF11-OE lines (n = 113, 72, 51, 53 biological independent plants for DN50, GmSGF11-OE-1, GmSGF11-OE-2 and GmSGF11-OE-3, respectively). h Comparison of seed width between DN50 and GmENY2-OE lines. Scale bars: 1 cm. i Seed width of DN50 and GmENY2-OE lines (n = 120, 30 biological independent plants for DN50 and GmENY2-OE lines, respectively). j 100-seed weight of DN50 and GmENY2-OE lines (n = 113, 42, 57 biological independent plants for DN50, GmENY2-OE-1 and GmENY2-OE-2, respectively). Experiment repeated two times with similar results in (b, d). In all the box plots, the center line indicates the median, the edges of the box represent the first and third quartiles, and the whiskers extend to the smallest and largest data points within 1.5 interquartile ranges from the edges. Statistical significance is determined using a two-sided t-test. Source data are provided as a Source Data file.
