Fig. 5: GmSW17 represses the expression of GmDP-E2F-1 by altering the level of H2Bub on GmDP-E2F-1.

a Volcano plot of DEGs in GmSW17^CR2/WT. b Relative expression level of GmDP-E2F-1 in DN50 and GmSW17 transgenic lines (n = 3 biological replicates). c H2Bub ChIP-seq track of GmDP-E2F-1 locus in DN50 and GmSW17^CR2. d H2Bub ChIP-qPCR validation of GmDP-E2F-1 locus in DN50 and GmSW17^CR2 (P1, P2 and P3). P1 to P3 represent regions covered by the primers used to assess H2Bub level by ChIP-qPCR. The data are normalized to the input chromatin, with GmTubulin used as a negative control. H2Bub levels in the WT are set to 1 (n = 3 biological replicates). e Cell numbers in the cell cycle phases with 2C (left) and 4C (right) nuclei in WT and GmSW17^CR lines are measured by flow cytometry (n = 3 biological replicates). f, Percentage of cells in different phases of the cell cycle in WT and GmSW17^CR seedlings (n = 3 biological replicates). ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant. g cryoSEM image of the cotyledon surface of DN50 and GmSW17 transgenic lines. Scale bars, 20 μm. h Cotyledon areas of DN50 and GmSW17 transgenic lines. i Cell area in the cotyledons of DN50 and GmSW17 transgenic lines. j Cell numbers on the ventral surface of DN50 and GmSW17 transgenic lines. The data in (b, d–f) are presented as the means ± SD. In all the box plots, the center line indicates the median, the edges of the box represent the first and third quartiles, and the whiskers extend to the smallest and largest data points within 1.5 interquartile ranges from the edges. Statistical significance is determined using a two-sided t-test. Source data are provided as a Source Data file.