Fig. 3: Liposarcoma-released IL-6 reprograms distant muscles.
From: A metabolic crosstalk between liposarcoma and muscle sustains tumor growth
A Proteome Profiler Human Cytokine Array. B Real-time qPCR analysis of IL-6 mRNA level of different cancer cell lines, SKOV3, HPAC, ZR-75.1, CFPAC-1, including LPS cell lines, IB115 and IB111. (n = 3 experimental replicates). C IL6 concentration (ng/ml), measured by ELISA, of different cancer cell lines, SKOV3, HPAC, ZR-75.1, CFPAC-1, including LPS cell lines, IB115 and IB111. (n = 3 experimental replicates). D End point of proliferation assay performed on XG-6 cells grown in media supplemented with the vehicle, recombinant IL-6, IB115, IB111, or MCF7 conditioned media. (n = 3 experimental replicates). E qChIP experiments showing the relative amounts of MDM2 and ATF4 on the IL-6 promoter in IB115 cells. Results were represented as the relative ratio between the mean value of immunoprecipitated chromatin (calculated as a percentage of the input) with the indicated antibodies and one control irrelevant antibody. (n = 3 experimental replicates). F Real-time qPCR analysis of IL-6 mRNA level in IB115 cells after shScr, shMdm2, shAtf4 or shIL-6 lentiviral infection, puromycin selection (48 h, 2μg/mL). (n = 3 experimental replicates). G IL6 concentration (ng/ml), measured by ELISA, of IB115 cells after shScr, shMdm2, shAtf4 or shIL-6 lentiviral infection, puromycin selection (48 h, 2μg/mL). (n = 3 experimental replicates). H Real-time qPCR analysis performed on C2C12 cells grown in media supplemented with vehicle or recombinant IL6 (50 pg/mL), evaluating the expression of serine synthesis pathway genes: Phgdh, Psat1, and Psph. (n = 8 experimental replicates). I, Real-time qPCR analysis performed on IB115 cells after shScr or shIL-6 cocultured with C2C12 cells (ratio 1:2), evaluating the expression of serine synthesis pathway genes: Phgdh, Psat1, and Psph. (n = 3 experimental replicates). J, Real-time qPCR analysis performed on C2C12 cells after shScr or shIL-6Rα cocultured with IB115 cells (ratio 1:2), evaluating the expression of serine synthesis pathway genes: Phgdh, Psat1, and Psph. (n = 13 experimental replicates). Data are shown as means ± SEM and three or more independent experiments were performed. Statistical tests were all adjusted for multiple comparisons and included two-tailed unpaired t-test for (h), one-way analysis of variance (ANOVA) for b, c, d, e, f, g, I, and j. *P < 0.05, **P < 0.01, ***<0.001, n.s non-significant. Source data are provided as a Source Data file.
