Fig. 5: Epigenetic age predictions in blood at single-cell resolution.

A We calculated the deviation between the real chronological age and the DNAme age predictions of our scEpiAge model for each single cell. Depicted are the deviations for each age group in violin plots and boxplots. The colours represent the different ages (matched to Fig. 1D). B For each cell, we estimated the empirical false discovery rate and plotted these against the age deviations between chronological and DNAme age. Individual cells are coloured by cell type: blue B-cell, orange CD4+ T-cell, green CD8+ T-cell, black other. C, D Number of expressed genes in cells with a DNAme age below 77 weeks or DNAme age above 101 weeks, showing cells from C all chronological age groups or D only from chronological age 101 weeks. The shade of red indicates the age groups of the cells. E Epigenetic age predictions per major cell type, grouped by chronological age (blue B-cell, orange CD4+ T-cell, green CD8+ T-cell, the shading represents age (darker younger, brighter older)). The boxplots in A, C–E show median levels and the first and third quartile, whiskers show 1.5× the interquartile range. Statistics shown in 5c&d from a linear model testing the number of expressed genes over 101 weeks or under, when correcting for read depth and cell type.