Fig. 2: Spatiotemporal dynamics of biomolecular condensates reveal the influences of diffusiophoresis on their formation, transport, and dissolution.
From: Diffusiophoresis promotes phase separation and transport of biomolecular condensates
Kymographs of the fluorescence signal of the [dT]40-[RGRGG]5 condensates without the presence of NaCl gradient [(a) c1 = c2 = 20 mM; (b) c1 = c2 = 1 mM]. The waves of the condensate are formed due to simultaneous dissolution near the front and condensation near the rear. c With NaCl gradients (c1 = 20 mM, c2 = 1 mM), the formation of the droplets with the migration toward the higher salt concentration delays the condensates from dissolution. The color code represents the fluorescence intensity I. d The trajectories of the individual condensates found near the front (x/L ~ 0.31, area 1) and rear (x/L ~ 0.26, area 2) of the wave in (c). The color code represents the velocity magnitude \(\left|{{{\boldsymbol{u}}}}\right|\). \(\left\langle \left|{{{\boldsymbol{u}}}}\right|\right\rangle={\sum }_{i=1}^{N}\int |{{{{\boldsymbol{u}}}}}_{{{{\boldsymbol{i}}}}}(t)|{dt}/N\) represents the mean of the time-averaged velocity magnitude of multiple condensates in each region. Source data are provided as a Source Data file.
