Fig. 3: Transcriptome and Functional Pathway Enrichment for day 5 dormant parasites (d5MDPs).
a A heatmap showing expression for genes (rep 1, gdown and gup in the same order as Fig. 2a) during the persistence phase (Day 6-7). Yellow color depicts higher FPKM values whereas blue depicts lower FPKM. b A boxplot depicting average expression (in log2FPKM, rep 1) of pathways on Day 5–7 was found to be significantly enriched during Gene Set Enrichment Analysis (GSEA) of d5MDP whole transcriptome. The boxplots are color-coded and categorized according to cellular/metabolic function similarities within the parasite (table on right). Each box represents the average expression values from first to third quartile with the black horizontal line across each box representing the median value. The top and bottom whiskers represent the maximum and minimum values of average expression (within the 1.5 times interquartile range) respectively. c A bubble plot depicting whole transcriptome correlations (Spearman’s Rank Correlation Coefficients, SPCC) for Day 5, 6, and 7 persistence phase of the parasite transcriptome (biological replicates - rep 1 and rep 2). d RT-PCR-based measurements of relative mRNA levels of selected genes between rings and d5MDPs. The error bars represent measurements between two biological replicas and three technical replicas from each (n = 6). Source data are provided as a Source Data file.
