Fig. 5: Viability evaluation using Propidium Iodine (PI) and Drug sensitivity profiling of P. falciparum d5MDPs. | Nature Communications

Fig. 5: Viability evaluation using Propidium Iodine (PI) and Drug sensitivity profiling of P. falciparum d5MDPs.

From: The artemisinin-induced dormant stages of Plasmodium falciparum exhibit hallmarks of cellular quiescence/senescence and drug resilience

Fig. 5

a FACS plots representing Hoechst 33342 and PI staining on Day 0 (6–12hpi rings) and Day 5 (d5MDPs) before and after paraformaldehyde + glutaraldehyde (PFA/GA) treatment. Live cells are indicated as Hoechst + /PI- in Q1 and dead cells are shown in Q2 (Hoechst + /PI + ). The percentage of infected live or dead RBCs in Q1 and Q2 respectively was calculated as: Qx% = Qx/(Q1 + Q2)*100 (where Qx Q1 for live and Qx Q2 for dead infected RBCs). Histograms show the PI staining shift before and after paraformaldehyde/glutaraldehyde (PFA/GA) treatment on the corresponding day. This experiment was performed once. b A graph showing recrudescence delay for d5MDPs treated with various antimalarial drugs/compounds for 24 h (started on Day 4) as compared to the controls (i.e. parasites treated with the corresponding drug solvent – DMSO, methanol or water, see Fig.S11A). Three different concentrations (i.e. 1xIC50, 10xIC50, 100 x IC50) were tested per drug. Three independent biological replicates were conducted with two technical replicates per condition. Error bars indicate SEM calculated from all the cultures/wells that eventually recovered. The circular charts below show the number of cultures/wells that recovered, died (i.e. no recovery up to 30 days) or were lysed for each drug concentration tested (legend on the right). Source data are provided as a Source Data file.

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