Fig. 2: PK of infused bNAbs and ADA responses. | Nature Communications

Fig. 2: PK of infused bNAbs and ADA responses.

From: Administration of anti-HIV-1 broadly neutralizing monoclonal antibodies with increased affinity to Fcγ receptors during acute SHIVAD8-EO infection

Fig. 2

A Concentration of VRC07-523-LS (dark pink), PGT121 (dark blue), VRC07-523-LS/DEL (light pink), and PGT121/DEL (light blue), as measured by ELISA in the plasma of monkeys that received either the WT or DEL bNAbs at days 3, 10, and 17 post-SHIVAD8-EO challenge. Vertical dotted lines indicate the timings of bNAb infusions and horizontal dotted lines indicate the in vitro neutralization IC80 values against SHIVAD8-EO averaged for VRC07-523-LS and VRC07-523-LS/DEL (pink), and PGT121 and PGT121/DEL (blue). N = 10 for each bNAb and each timepoint except for VRC07-523-LS and PGT121 at week 0 and VRC07-523-LS/DEL and PGT121/DEL at weeks 10–14: n = 4; PGT121 at week 9: n = 9. B Plasma concentration of each infused bNAb up to 14 weeks post-challenge as determined by AUC analysis. N = 10 for each bNAb. Each P < 0.0001, two-sided p-values. C ADA response against VRC07-523-LS (dark pink), PGT121 (dark blue), VRC07-523-LS/DEL (light pink), and PGT121/DEL (light blue), as measured by ELISA in the plasma of monkeys that received either the WT or DEL bNAbs at days 3, 10, and 17 post-SHIVAD8-EO challenge. Vertical dotted lines indicate the timings of bNAb infusions. N = 10 for each bNAb and each timepoint except for α-VRC07-523-LS at weeks 0 and 1, α-PGT121 at week 0, and α-VRC07-523-LS/DEL at week 6: n = 9; α-VRC07-523-LS/DEL at week 4: n = 8; α-VRC07-523-LS at days 10 and 17 and α-PGT121 at days 3, 10, and 17: n = 4, and α-VRC07-523-LS at day 3: n = 3. D ADA response against each infused bNAb up to 14 weeks post-challenge as determined by AUC analysis. N = 10 for each bNAb. P = 0.0089 for α-VRC07-523-LS vs. α-VRC07-523-LS/DEL and p = 0.0029 for α-PGT121 vs. α-PGT121/DEL, two-sided p-values. Graphs show the mean±SEM (A, C) and the mean and individual datapoints (B, D). The Mann-Whitney test was used to detect significant differences between the WT bNAbs, the DEL bNAbs, or the WT and DEL mutant of the same bNAb. N indicates the number of biological replicates (monkeys). Source data are provided in the Source Data file. ADA anti-drug antibody; AUC area under the curve.

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