Fig. 5: SIV Gag-specific CD8+ T cell responses. | Nature Communications

Fig. 5: SIV Gag-specific CD8+ T cell responses.

From: Administration of anti-HIV-1 broadly neutralizing monoclonal antibodies with increased affinity to Fcγ receptors during acute SHIVAD8-EO infection

Fig. 5

A Frequency of SIV Gag-specific CD8+ T cells in PBMCs (left) and LN cells (right) from monkeys that were left untreated or were treated with bNAbs early after SHIVAD8-EO challenge. N = 9 untreated monkeys except for LN cells pre: n = 7, week 2: n = 6, and week 20: n = 4. N = 8 and 7 WT and DEL bNAb-treated monkeys, respectively. P = 0.0474, 0.0480, and 0.0053 for untreated vs. WT group at week 4, 6, and 8, respectively, and p = 0.0485 and 0.0408 for WT vs. DEL group at weeks 6 and 8, respectively (left) (B, C) Polyfunctional profile of SIV Gag-specific CD8+ T cell responses in PBMCs (top) and LN cells (bottom) at week 20 post-challenge. N = 9 and 4 untreated monkeys for PBMCs and LN cells, respectively; n = 8 and 7 WT and DEL bNAb-treated monkeys, respectively. B P = 0.04 for WT vs. DEL group in PBMCs; p < 0.001 for PBMCs vs. LN cells in untreated and DEL groups, and p = 0.002 for PBMCs vs. LN cells in WT group; two-sided p-values. C PBMCs, IFNγ+MIP-1β+TNF-CD107a- CD8+ T cells: p = 0.0099 and 0.0014 for untreated vs. DEL and WT vs. DEL group, respectively. PBMCs, IFNγ+MIP-1β-TNF-CD107a- CD8+ T cells: p = 0.0209 and 0.0240 for untreated vs. DEL and WT vs. DEL group, respectively. LN cells, IFNγ-MIP-1β+TNF+CD107a+ CD8+ T cells: p = 0.0333 for untreated vs. WT group. Graphs show the mean (A–C) and individual datapoints. The Kruskal–Wallis test followed by Dunn’s multiple comparison test was used to detect significant differences between monkey groups at each timepoint (A) and for each functional readout combination (C), and between timepoints for each monkey group (A, colored statistics). Statistics in gray, orange, and green, denote, respectively, differences from week 2 for untreated monkeys, week 4 for WT bNAb-treated monkeys, and pre-challenge for DEL bNAb-treated monkeys in PBMCs (A, left), and from week 20 in LN cells (A, right). The permutation test was used to compare pie charts (B). N indicates the number of biological replicates (monkeys). Source data are provided in the Source Data file. LN lymph node, PBMCs peripheral blood mononuclear cells, pre pre-challenge, Tx treatment, UnTx untreated, wk week.

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