Fig. 1: Outline and cell type characterization.

a Overview of methodology, pgWAT from both female (n = 5) and male (n = 3) mice. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). b Seurat clustering of complete dataset (17 clusters) and UMAP dimensional reduction visualization. c Hierarchical ordering of clusters based on Pearson’s r value of the scaled average expression of marker gene expression in each cluster with cell-type annotation of clusters and dotplot of selected marker genes. The 45-gene signature for mural and fibroblasts, respectively, as defined by Muhl et al.²⁵, are displayed in the dotplot. d Organotypic matrisome genes in fibroblasts from pgWAT, heart and skeletal muscle. e Enriched molecular functions in adipose ASC when compared to fibroblasts from heart and skeletal muscle, barplot shows gene expression of the enriched genes involved in each function. Red mark in the spreadsheet means that the gene is represented in the molecular function and white mark means that it is not represented. f Dotplot over adipogenic gene features of fibroblasts/ASC versus mural cells in pgWAT. g Barplots over common stem cells markers used to identify mesenchymal stem cells. Data are presented as mean values +/- SEM. n represent number of cells for scRNA-seq data. Abbreviations: ASC= Adipose stem cell, EpC = Epithelial cells, EC = Endothelial cells, LEC = Lymphatic EC, MAC = Macrophages, Mito=mitochondrial, pg= perigonadal white adipose tissue, SEM=Standard error of the mean,UMAP=Uniform Manifold Approximation and Projection and VSMC = Vascular smooth muscle cells.