Fig. 3: RAAS and glucose metabolism disorder two sexually dimorphic pathways. | Nature Communications

Fig. 3: RAAS and glucose metabolism disorder two sexually dimorphic pathways.

From: Adipose stem cells are sexually dimorphic cells with dual roles as preadipocytes and resident fibroblasts

Fig. 3

a Enriched canonical pathways in male and female ASC based on the core set of 36 sexually dimorphic genes. P-Values are derived from IPA-analysis and based on the right-tailed Fisher’s Test. b Schematic cartoon over the RAAS-system and the expression of its main components in adipose tissue. Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). c Barplots of the expression of RAAS-associated genes in scRNA-seq dataset, FACS sorted ASC populations (bulk RNA-seq) and mature adipocytes (bulk RNA-seq). For all bulk samples n = 7 biological replicates except ASC1 male iWAT and mature adipocytes samples (n = 8). d AngII effect on lipolysis from iWAT explants. n = 3 biological replicates, P-value = 0.0092 e AngII effect on in vitro differentiation of crude SVF cells from iWAT. n = 5 and represent five independent experiments. f Expression of detected RAAS-associated genes in cultivated SVF cells prior to initiation of differentiation. n = 9 technical well replicates from three independent experiments g Expression of Esr1 in ASC from pgWAT (scRNA-seq data). Statistics in Fig. 3d, e were calculated with two-way Anova using Sidak’s multiple comparisons test. Data are presented as mean values +/- SEM for c, f,g and mean values +/- SD for d and e. AngII Angiotensin II, ASC Adipose stem cells, ETC electron transport chain, i inguinal, pg perigonadal, RAAS Renin-angiotensin-aldosterone system, sc single cell, SD standard deviation, SEM Standard error of the mean, seq sequencing and WAT White adipose tissue.

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